n Example of the Use of Loci Information
On the gel, we see the bands for the daughters and foundresses from colony114 at locus Pbe 424AAT. Though we do not have any genetic information from the fathers, in this species, each mother mates with only one male, so all of the daughters of one mother will have a shared band from the father. In this case, all of the daughters have a 207 band. Since neither of the foundresses have this band, we can assume that each of these daughters got this from her father. Furthermore, because they all have this same shared band, it is likely that all of the daughters has the same father, however, it is important to verify this with information from other loci. Now, daughters 10, 12, 13, 15, and 16 have a band at 204, as does foundress 20. Because foundress 19 does not have a 204 band, we can assume that foundress 20 is the mother of those daughters. Because it is likely that all of the daughters have the same father, we can guess that foundress 20 is the mother of all of the duahgters. However, the remaining daughters have band 210, which both of the foundresses have. Therefore, if we do not assume that all of the daughters have the same father, from this locus, we cannot determine the mother of those remaining daughters from the information provided by this locus. It is necessary to confirm results with and to gain further from other loci.
When determining parentage, it is important to remember not to assume too much. Remember,